Transferability makes the black-box attacks to be practical. Recent studies demonstrate that adversarial examples situated at the flat maxima on the loss landscape tend to exhibit higher transferability and propose effective strategies to optimize adversarial examples to converge toward that region. However, these works primarily consider the first-order gradient regularization and have yet to explore higher-order geometry properties of the flat loss landscape, which may lead to suboptimal results. In this work, we propose leveraging the trace of the Hessian matrix of loss function with respect to the adversarial example as a curvature-aware regularizer. For computationally efficient, we introduce an approximation method for the trace based on stochastic estimation and finite difference. We theoretically and empirically demonstrate that the trace of Hessian matrices for adversarial examples near local loss maxima is consistently negative. Following this insight, we propose Negative Hessian Trace Regularization (NHTR), explicitly penalizing the negative Hessian trace to suppress curvature. Compared to existing first-order regularization methods, NHTR can generate adversarial examples at flatter local regions. Extensive experimental results on the ImageNet-compatible and CIFAR-10 datasets show that NHTR can significantly improve adversarial transferability than the state-of-the-art attacks.