Bio-image computing (BIC) is a rapidly growing field at the interface of engineering, biology and computer science. Advanced light microscopy can deliver 2D and 3D image sequences of living cells with unprecedented image quality and ever increasing resolution in space and time. The emergence of novel and diverse microscopy modalities has provided biologists with unprecedented means to explore cellular mechanisms, embryogenesis, and neural development, to mention only a few fundamental biological questions. The enormous size and complexity of these data sets, which can exceed multiple TB per volume or video, requires state-of-the-art computer vision methods.